Characterization of a reference material for BCR-ABL (M-BCR) mRNA quantitation by real-time amplification assays : towards new standards for gene expression measurements
Identifieur interne : 009686 ( Main/Exploration ); précédent : 009685; suivant : 009687Characterization of a reference material for BCR-ABL (M-BCR) mRNA quantitation by real-time amplification assays : towards new standards for gene expression measurements
Auteurs : J. Saldanhal [Royaume-Uni, France, Australie] ; M. Silvy [France] ; N. Beaufils [France] ; R. Arlinghaus [États-Unis] ; G. Barbany [Suède] ; S. Branford [Australie] ; J.-M. Cayuela [France] ; G. Cazzaniga [Italie] ; M. Gonzalez [Espagne] ; D. Grimwade [Royaume-Uni] ; V. Kairisto [Finlande] ; K. Miyamura [Japon] ; M. Lawler [Irlande (pays)] ; T. Lion [Autriche] ; E. Macintyre [France] ; F.-X. Mahon [France] ; M. C. Muller [Allemagne] ; M. Ostergaard [Danemark] ; H. Pfeifer [Allemagne] ; G. Saglio [Italie] ; C. Sawyers [États-Unis] ; O. Spinelli [Italie] ; V. H. J. Van Der Velden [Pays-Bas] ; J. Q. Wang [États-Unis] ; K. Zoi [Grèce] ; V. Patel [Royaume-Uni] ; P. Phillips [Royaume-Uni] ; P. Matejtschuk [Royaume-Uni] ; J. Gabert [France]Source :
- Leukemia [ 0887-6924 ] ; 2007.
Descripteurs français
- Pascal (Inist)
- Réaction chaîne polymérase, Matériau référence, Gène hybride, Translocation chromosomique, Chromosome C9 anormal, Chromosome G22 anormal, Chromosome Ph1, Protein kinase, RNA messager, Temps réel, Amplification, Norme, Expression génique, Gel, Normalisation, Hématologie, Gène bcr, Gène abl, Protéine bcr-abl.
- Wicri :
- topic : Norme, Normalisation.
English descriptors
- KwdEn :
Abstract
Monitoring of BCR-ABL transcripts has become established practice in the management of chronic myeloid leukemia. However, nucleic acid amplification techniques are prone to variations which limit the reliability of real-time quantitative PCR (RQ-PCR) for clinical decision making, highlighting the need for standardization of assays and reporting of minimal residual disease (MRD) data. We evaluated a lyophilized preparation of a leukemic cell line (K562) as a potential quality control reagent. This was found to be relatively stable, yielding comparable respective levels of ABL, GUS and BCR-ABL transcripts as determined by RQ-PCR before and after accelerated degradation experiments as well as following 5 years storage at -20°C. Vials of freeze-dried cells were sent at ambient temperature to 22 laboratories on four continents, with RQ-PCR analyses detecting BCR-ABL transcripts at levels comparable to those observed in primary patient samples. Our results suggest that freeze-dried cells can be used as quality control reagents with a range of analytical instrumentations and could enable the development of urgently needed international standards simulating clinically relevant levels of MRD.
Affiliations:
- Allemagne, Australie, Autriche, Danemark, Espagne, Finlande, France, Grèce, Irlande (pays), Italie, Japon, Pays-Bas, Royaume-Uni, Suède, États-Unis
- Angleterre, Aquitaine, Attique (région), Bade-Wurtemberg, District de Darmstadt, District de Karlsruhe, Finlande occidentale, Grand Londres, Hesse (Land), Hollande-Méridionale, Nouvelle-Aquitaine, Piémont, Provence-Alpes-Côte d'Azur, Svealand, Vienne (Autriche), Île-de-France
- Athènes, Bordeaux, Francfort-sur-le-Main, Londres, Mannheim, Marseille, Paris, Rotterdam, Stockholm, Turin, Turku, Vienne (Autriche)
- Université de la Méditerranée
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Beaufils</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Biochemistry and Molecular Biology Laboratory ERT MEIDIA, APHM-IFR Jean Roche, Université de la Méditerranée</s1><s2>Marseille</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>29 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université de la Méditerranée</orgName></affiliation></author><author><name sortKey="Arlinghaus, R" sort="Arlinghaus, R" uniqKey="Arlinghaus R" first="R." last="Arlinghaus">R. Arlinghaus</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Molecular Pathology, MD Anderson</s1><s2>Houston, TX</s2><s3>USA</s3><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Houston, TX</wicri:noRegion></affiliation></author><author><name sortKey="Barbany, G" sort="Barbany, G" uniqKey="Barbany G" first="G." last="Barbany">G. Barbany</name><affiliation wicri:level="3"><inist:fA14 i1="05"><s1>Molecular Medicine and Surgery, Karolinska Hospital</s1><s2>Stockholm</s2><s3>SWE</s3><sZ>5 aut.</sZ></inist:fA14><country>Suède</country><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation></author><author><name sortKey="Branford, S" sort="Branford, S" uniqKey="Branford S" first="S." last="Branford">S. Branford</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Molecular Pathology, Institute of Medicine & Veterinary Science</s1><s2>Adelaide</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Adelaide</wicri:noRegion></affiliation></author><author><name sortKey="Cayuela, J M" sort="Cayuela, J M" uniqKey="Cayuela J" first="J.-M." last="Cayuela">J.-M. Cayuela</name><affiliation wicri:level="3"><inist:fA14 i1="06"><s1>Hematology and INSERM U728, St Louis Hospital</s1><s2>Paris</s2><s3>FRA</s3><sZ>7 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Cazzaniga, G" sort="Cazzaniga, G" uniqKey="Cazzaniga G" first="G." last="Cazzaniga">G. Cazzaniga</name><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Pediatric Clinic, University of Milan-Bicocca, Ospedale San Gerardo</s1><s2>Monza</s2><s3>ITA</s3><sZ>8 aut.</sZ></inist:fA14><country>Italie</country><wicri:noRegion>Monza</wicri:noRegion></affiliation></author><author><name sortKey="Gonzalez, M" sort="Gonzalez, M" uniqKey="Gonzalez M" first="M." last="Gonzalez">M. Gonzalez</name><affiliation wicri:level="1"><inist:fA14 i1="08"><s1>Hematology, Hospital Clinico Universitario</s1><s2>Salamanca</s2><s3>ESP</s3><sZ>9 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>Salamanca</wicri:noRegion></affiliation></author><author><name sortKey="Grimwade, D" sort="Grimwade, D" uniqKey="Grimwade D" first="D." last="Grimwade">D. Grimwade</name><affiliation wicri:level="3"><inist:fA14 i1="09"><s1>Department of Medical & Molecular Genetics, King's College London</s1><s2>London</s2><s3>GBR</s3><sZ>10 aut.</sZ></inist:fA14><country>Royaume-Uni</country><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Kairisto, V" sort="Kairisto, V" uniqKey="Kairisto V" first="V." last="Kairisto">V. Kairisto</name><affiliation wicri:level="3"><inist:fA14 i1="10"><s1>Laboratory Department 931, TYKSLAB, Turku University Hospital</s1><s2>Turku</s2><s3>FIN</s3><sZ>11 aut.</sZ></inist:fA14><country>Finlande</country><placeName><settlement type="city">Turku</settlement><region type="région" nuts="2">Finlande occidentale</region></placeName></affiliation></author><author><name sortKey="Miyamura, K" sort="Miyamura, K" uniqKey="Miyamura K" first="K." last="Miyamura">K. Miyamura</name><affiliation wicri:level="1"><inist:fA14 i1="11"><s1>Rheumatology and Hematology, Tohoku University School of Medicine1</s1><s2>Sendai</s2><s3>JPN</s3><sZ>12 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sendai</wicri:noRegion></affiliation></author><author><name sortKey="Lawler, M" sort="Lawler, M" uniqKey="Lawler M" first="M." last="Lawler">M. Lawler</name><affiliation wicri:level="1"><inist:fA14 i1="12"><s1>Hematology, St James's Hospital</s1><s2>Dublin</s2><s3>IRL</s3><sZ>13 aut.</sZ></inist:fA14><country>Irlande (pays)</country><wicri:noRegion>Dublin</wicri:noRegion></affiliation></author><author><name sortKey="Lion, T" sort="Lion, T" uniqKey="Lion T" first="T." last="Lion">T. Lion</name><affiliation wicri:level="3"><inist:fA14 i1="13"><s1>Molecular Biology, Children's Cancer Research Institute</s1><s2>Vienna</s2><s3>AUT</s3><sZ>14 aut.</sZ></inist:fA14><country>Autriche</country><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Macintyre, E" sort="Macintyre, E" uniqKey="Macintyre E" first="E." last="Macintyre">E. Macintyre</name><affiliation wicri:level="3"><inist:fA14 i1="14"><s1>Hematology, Necker Hospital</s1><s2>Paris</s2><s3>FRA</s3><sZ>15 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Mahon, F X" sort="Mahon, F X" uniqKey="Mahon F" first="F.-X." last="Mahon">F.-X. Mahon</name><affiliation wicri:level="3"><inist:fA14 i1="15"><s1>Laboratoire de greffe de Moelle -UMR 5540, University Hospital</s1><s2>Bordeaux</s2><s3>FRA</s3><sZ>16 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Nouvelle-Aquitaine</region><region type="old region">Aquitaine</region><settlement type="city">Bordeaux</settlement></placeName></affiliation></author><author><name sortKey="Muller, M C" sort="Muller, M C" uniqKey="Muller M" first="M. C." last="Muller">M. C. Muller</name><affiliation wicri:level="3"><inist:fA14 i1="16"><s1>III Medizinische Klinik, Medizinische Fakultaet Mannheim der Universitaet Heidelberg</s1><s2>Mannheim</s2><s3>DEU</s3><sZ>17 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Mannheim</settlement></placeName></affiliation></author><author><name sortKey="Ostergaard, M" sort="Ostergaard, M" uniqKey="Ostergaard M" first="M." last="Ostergaard">M. Ostergaard</name><affiliation wicri:level="1"><inist:fA14 i1="17"><s1>Department of Hematology, Aarhus University Hospital</s1><s2>Aarhus</s2><s3>DNK</s3><sZ>18 aut.</sZ></inist:fA14><country>Danemark</country><wicri:noRegion>Aarhus</wicri:noRegion></affiliation></author><author><name sortKey="Pfeifer, H" sort="Pfeifer, H" uniqKey="Pfeifer H" first="H." last="Pfeifer">H. Pfeifer</name><affiliation wicri:level="3"><inist:fA14 i1="18"><s1>Hematology, University Hospital</s1><s2>Frankfurt</s2><s3>DEU</s3><sZ>19 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Darmstadt</region><settlement type="city">Francfort-sur-le-Main</settlement></placeName></affiliation></author><author><name sortKey="Saglio, G" sort="Saglio, G" uniqKey="Saglio G" first="G." last="Saglio">G. Saglio</name><affiliation wicri:level="3"><inist:fA14 i1="19"><s1>Clinical and Biological Science, University Hospital</s1><s2>Turin</s2><s3>ITA</s3><sZ>20 aut.</sZ></inist:fA14><country>Italie</country><placeName><settlement type="city">Turin</settlement><region type="région" nuts="2">Piémont</region></placeName></affiliation></author><author><name sortKey="Sawyers, C" sort="Sawyers, C" uniqKey="Sawyers C" first="C." last="Sawyers">C. Sawyers</name><affiliation wicri:level="1"><inist:fA14 i1="20"><s1>Hematology Oncology, UCLA</s1><s2>Los Angeles, CA</s2><s3>USA</s3><sZ>21 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Los Angeles, CA</wicri:noRegion></affiliation></author><author><name sortKey="Spinelli, O" sort="Spinelli, O" uniqKey="Spinelli O" first="O." last="Spinelli">O. Spinelli</name><affiliation wicri:level="1"><inist:fA14 i1="21"><s1>Hematology, Ospedali Riuniti</s1><s2>Bergamo</s2><s3>ITA</s3><sZ>22 aut.</sZ></inist:fA14><country>Italie</country><wicri:noRegion>Bergamo</wicri:noRegion></affiliation></author><author><name sortKey="Van Der Velden, V H J" sort="Van Der Velden, V H J" uniqKey="Van Der Velden V" first="V. H. J." last="Van Der Velden">V. H. J. Van Der Velden</name><affiliation wicri:level="3"><inist:fA14 i1="22"><s1>Immunology, Erasmus, MC University Medical Center Rotterdam</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>23 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Wang, J Q" sort="Wang, J Q" uniqKey="Wang J" first="J. Q." last="Wang">J. Q. Wang</name><affiliation wicri:level="1"><inist:fA14 i1="23"><s1>Specialty Laboratories</s1><s2>Santa Monica, CA</s2><s3>USA</s3><sZ>24 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Specialty Laboratories</wicri:noRegion></affiliation></author><author><name sortKey="Zoi, K" sort="Zoi, K" uniqKey="Zoi K" first="K." last="Zoi">K. Zoi</name><affiliation wicri:level="3"><inist:fA14 i1="24"><s1>Foundation for Biomedical Research, Academy of Athens</s1><s2>Athens</s2><s3>GRC</s3><sZ>25 aut.</sZ></inist:fA14><country>Grèce</country><placeName><settlement type="city">Athènes</settlement><region nuts="2" type="region">Attique (région)</region></placeName></affiliation></author><author><name sortKey="Patel, V" sort="Patel, V" uniqKey="Patel V" first="V." last="Patel">V. Patel</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute of Biological Standards and Controls</s1><s2>South Mimms</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>National Institute of Biological Standards and Controls</wicri:noRegion></affiliation></author><author><name sortKey="Phillips, P" sort="Phillips, P" uniqKey="Phillips P" first="P." last="Phillips">P. Phillips</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute of Biological Standards and Controls</s1><s2>South Mimms</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>National Institute of Biological Standards and Controls</wicri:noRegion></affiliation></author><author><name sortKey="Matejtschuk, P" sort="Matejtschuk, P" uniqKey="Matejtschuk P" first="P." last="Matejtschuk">P. Matejtschuk</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute of Biological Standards and Controls</s1><s2>South Mimms</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>National Institute of Biological Standards and Controls</wicri:noRegion></affiliation></author><author><name sortKey="Gabert, J" sort="Gabert, J" uniqKey="Gabert J" first="J." last="Gabert">J. Gabert</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Biochemistry and Molecular Biology Laboratory ERT MEIDIA, APHM-IFR Jean Roche, Université de la Méditerranée</s1><s2>Marseille</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>29 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université de la Méditerranée</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">07-0337536</idno><date when="2007">2007</date><idno type="stanalyst">PASCAL 07-0337536 INIST</idno><idno type="RBID">Pascal:07-0337536</idno><idno type="wicri:Area/PascalFrancis/Corpus">003B22</idno><idno type="wicri:Area/PascalFrancis/Curation">002566</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">003A16</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">003A16</idno><idno type="wicri:doubleKey">0887-6924:2007:Saldanhal J:characterization:of:a</idno><idno type="wicri:Area/Main/Merge">00A128</idno><idno type="wicri:Area/Main/Curation">009686</idno><idno type="wicri:Area/Main/Exploration">009686</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Characterization of a reference material for BCR-ABL (M-BCR) mRNA quantitation by real-time amplification assays : towards new standards for gene expression measurements</title><author><name sortKey="Saldanhal, J" sort="Saldanhal, J" uniqKey="Saldanhal J" first="J." last="Saldanhal">J. Saldanhal</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute of Biological Standards and Controls</s1><s2>South Mimms</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>National Institute of Biological Standards and Controls</wicri:noRegion></affiliation><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Biochemistry and Molecular Biology Laboratory ERT MEIDIA, APHM-IFR Jean Roche, Université de la Méditerranée</s1><s2>Marseille</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>29 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université de la Méditerranée</orgName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Molecular Pathology, Institute of Medicine & Veterinary Science</s1><s2>Adelaide</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Adelaide</wicri:noRegion></affiliation></author><author><name sortKey="Silvy, M" sort="Silvy, M" uniqKey="Silvy M" first="M." last="Silvy">M. Silvy</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Biochemistry and Molecular Biology Laboratory ERT MEIDIA, APHM-IFR Jean Roche, Université de la Méditerranée</s1><s2>Marseille</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>29 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université de la Méditerranée</orgName></affiliation></author><author><name sortKey="Beaufils, N" sort="Beaufils, N" uniqKey="Beaufils N" first="N." last="Beaufils">N. Beaufils</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Biochemistry and Molecular Biology Laboratory ERT MEIDIA, APHM-IFR Jean Roche, Université de la Méditerranée</s1><s2>Marseille</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>29 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université de la Méditerranée</orgName></affiliation></author><author><name sortKey="Arlinghaus, R" sort="Arlinghaus, R" uniqKey="Arlinghaus R" first="R." last="Arlinghaus">R. Arlinghaus</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Molecular Pathology, MD Anderson</s1><s2>Houston, TX</s2><s3>USA</s3><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Houston, TX</wicri:noRegion></affiliation></author><author><name sortKey="Barbany, G" sort="Barbany, G" uniqKey="Barbany G" first="G." last="Barbany">G. Barbany</name><affiliation wicri:level="3"><inist:fA14 i1="05"><s1>Molecular Medicine and Surgery, Karolinska Hospital</s1><s2>Stockholm</s2><s3>SWE</s3><sZ>5 aut.</sZ></inist:fA14><country>Suède</country><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation></author><author><name sortKey="Branford, S" sort="Branford, S" uniqKey="Branford S" first="S." last="Branford">S. Branford</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Molecular Pathology, Institute of Medicine & Veterinary Science</s1><s2>Adelaide</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Adelaide</wicri:noRegion></affiliation></author><author><name sortKey="Cayuela, J M" sort="Cayuela, J M" uniqKey="Cayuela J" first="J.-M." last="Cayuela">J.-M. Cayuela</name><affiliation wicri:level="3"><inist:fA14 i1="06"><s1>Hematology and INSERM U728, St Louis Hospital</s1><s2>Paris</s2><s3>FRA</s3><sZ>7 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Cazzaniga, G" sort="Cazzaniga, G" uniqKey="Cazzaniga G" first="G." last="Cazzaniga">G. Cazzaniga</name><affiliation wicri:level="1"><inist:fA14 i1="07"><s1>Pediatric Clinic, University of Milan-Bicocca, Ospedale San Gerardo</s1><s2>Monza</s2><s3>ITA</s3><sZ>8 aut.</sZ></inist:fA14><country>Italie</country><wicri:noRegion>Monza</wicri:noRegion></affiliation></author><author><name sortKey="Gonzalez, M" sort="Gonzalez, M" uniqKey="Gonzalez M" first="M." last="Gonzalez">M. Gonzalez</name><affiliation wicri:level="1"><inist:fA14 i1="08"><s1>Hematology, Hospital Clinico Universitario</s1><s2>Salamanca</s2><s3>ESP</s3><sZ>9 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>Salamanca</wicri:noRegion></affiliation></author><author><name sortKey="Grimwade, D" sort="Grimwade, D" uniqKey="Grimwade D" first="D." last="Grimwade">D. Grimwade</name><affiliation wicri:level="3"><inist:fA14 i1="09"><s1>Department of Medical & Molecular Genetics, King's College London</s1><s2>London</s2><s3>GBR</s3><sZ>10 aut.</sZ></inist:fA14><country>Royaume-Uni</country><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Kairisto, V" sort="Kairisto, V" uniqKey="Kairisto V" first="V." last="Kairisto">V. Kairisto</name><affiliation wicri:level="3"><inist:fA14 i1="10"><s1>Laboratory Department 931, TYKSLAB, Turku University Hospital</s1><s2>Turku</s2><s3>FIN</s3><sZ>11 aut.</sZ></inist:fA14><country>Finlande</country><placeName><settlement type="city">Turku</settlement><region type="région" nuts="2">Finlande occidentale</region></placeName></affiliation></author><author><name sortKey="Miyamura, K" sort="Miyamura, K" uniqKey="Miyamura K" first="K." last="Miyamura">K. Miyamura</name><affiliation wicri:level="1"><inist:fA14 i1="11"><s1>Rheumatology and Hematology, Tohoku University School of Medicine1</s1><s2>Sendai</s2><s3>JPN</s3><sZ>12 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Sendai</wicri:noRegion></affiliation></author><author><name sortKey="Lawler, M" sort="Lawler, M" uniqKey="Lawler M" first="M." last="Lawler">M. Lawler</name><affiliation wicri:level="1"><inist:fA14 i1="12"><s1>Hematology, St James's Hospital</s1><s2>Dublin</s2><s3>IRL</s3><sZ>13 aut.</sZ></inist:fA14><country>Irlande (pays)</country><wicri:noRegion>Dublin</wicri:noRegion></affiliation></author><author><name sortKey="Lion, T" sort="Lion, T" uniqKey="Lion T" first="T." last="Lion">T. Lion</name><affiliation wicri:level="3"><inist:fA14 i1="13"><s1>Molecular Biology, Children's Cancer Research Institute</s1><s2>Vienna</s2><s3>AUT</s3><sZ>14 aut.</sZ></inist:fA14><country>Autriche</country><placeName><settlement type="city">Vienne (Autriche)</settlement><region nuts="2" type="province">Vienne (Autriche)</region></placeName></affiliation></author><author><name sortKey="Macintyre, E" sort="Macintyre, E" uniqKey="Macintyre E" first="E." last="Macintyre">E. Macintyre</name><affiliation wicri:level="3"><inist:fA14 i1="14"><s1>Hematology, Necker Hospital</s1><s2>Paris</s2><s3>FRA</s3><sZ>15 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Mahon, F X" sort="Mahon, F X" uniqKey="Mahon F" first="F.-X." last="Mahon">F.-X. Mahon</name><affiliation wicri:level="3"><inist:fA14 i1="15"><s1>Laboratoire de greffe de Moelle -UMR 5540, University Hospital</s1><s2>Bordeaux</s2><s3>FRA</s3><sZ>16 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Nouvelle-Aquitaine</region><region type="old region">Aquitaine</region><settlement type="city">Bordeaux</settlement></placeName></affiliation></author><author><name sortKey="Muller, M C" sort="Muller, M C" uniqKey="Muller M" first="M. C." last="Muller">M. C. Muller</name><affiliation wicri:level="3"><inist:fA14 i1="16"><s1>III Medizinische Klinik, Medizinische Fakultaet Mannheim der Universitaet Heidelberg</s1><s2>Mannheim</s2><s3>DEU</s3><sZ>17 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Karlsruhe</region><settlement type="city">Mannheim</settlement></placeName></affiliation></author><author><name sortKey="Ostergaard, M" sort="Ostergaard, M" uniqKey="Ostergaard M" first="M." last="Ostergaard">M. Ostergaard</name><affiliation wicri:level="1"><inist:fA14 i1="17"><s1>Department of Hematology, Aarhus University Hospital</s1><s2>Aarhus</s2><s3>DNK</s3><sZ>18 aut.</sZ></inist:fA14><country>Danemark</country><wicri:noRegion>Aarhus</wicri:noRegion></affiliation></author><author><name sortKey="Pfeifer, H" sort="Pfeifer, H" uniqKey="Pfeifer H" first="H." last="Pfeifer">H. Pfeifer</name><affiliation wicri:level="3"><inist:fA14 i1="18"><s1>Hematology, University Hospital</s1><s2>Frankfurt</s2><s3>DEU</s3><sZ>19 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Darmstadt</region><settlement type="city">Francfort-sur-le-Main</settlement></placeName></affiliation></author><author><name sortKey="Saglio, G" sort="Saglio, G" uniqKey="Saglio G" first="G." last="Saglio">G. Saglio</name><affiliation wicri:level="3"><inist:fA14 i1="19"><s1>Clinical and Biological Science, University Hospital</s1><s2>Turin</s2><s3>ITA</s3><sZ>20 aut.</sZ></inist:fA14><country>Italie</country><placeName><settlement type="city">Turin</settlement><region type="région" nuts="2">Piémont</region></placeName></affiliation></author><author><name sortKey="Sawyers, C" sort="Sawyers, C" uniqKey="Sawyers C" first="C." last="Sawyers">C. Sawyers</name><affiliation wicri:level="1"><inist:fA14 i1="20"><s1>Hematology Oncology, UCLA</s1><s2>Los Angeles, CA</s2><s3>USA</s3><sZ>21 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Los Angeles, CA</wicri:noRegion></affiliation></author><author><name sortKey="Spinelli, O" sort="Spinelli, O" uniqKey="Spinelli O" first="O." last="Spinelli">O. Spinelli</name><affiliation wicri:level="1"><inist:fA14 i1="21"><s1>Hematology, Ospedali Riuniti</s1><s2>Bergamo</s2><s3>ITA</s3><sZ>22 aut.</sZ></inist:fA14><country>Italie</country><wicri:noRegion>Bergamo</wicri:noRegion></affiliation></author><author><name sortKey="Van Der Velden, V H J" sort="Van Der Velden, V H J" uniqKey="Van Der Velden V" first="V. H. J." last="Van Der Velden">V. H. J. Van Der Velden</name><affiliation wicri:level="3"><inist:fA14 i1="22"><s1>Immunology, Erasmus, MC University Medical Center Rotterdam</s1><s2>Rotterdam</s2><s3>NLD</s3><sZ>23 aut.</sZ></inist:fA14><country>Pays-Bas</country><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Wang, J Q" sort="Wang, J Q" uniqKey="Wang J" first="J. Q." last="Wang">J. Q. Wang</name><affiliation wicri:level="1"><inist:fA14 i1="23"><s1>Specialty Laboratories</s1><s2>Santa Monica, CA</s2><s3>USA</s3><sZ>24 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Specialty Laboratories</wicri:noRegion></affiliation></author><author><name sortKey="Zoi, K" sort="Zoi, K" uniqKey="Zoi K" first="K." last="Zoi">K. Zoi</name><affiliation wicri:level="3"><inist:fA14 i1="24"><s1>Foundation for Biomedical Research, Academy of Athens</s1><s2>Athens</s2><s3>GRC</s3><sZ>25 aut.</sZ></inist:fA14><country>Grèce</country><placeName><settlement type="city">Athènes</settlement><region nuts="2" type="region">Attique (région)</region></placeName></affiliation></author><author><name sortKey="Patel, V" sort="Patel, V" uniqKey="Patel V" first="V." last="Patel">V. Patel</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute of Biological Standards and Controls</s1><s2>South Mimms</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>National Institute of Biological Standards and Controls</wicri:noRegion></affiliation></author><author><name sortKey="Phillips, P" sort="Phillips, P" uniqKey="Phillips P" first="P." last="Phillips">P. Phillips</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute of Biological Standards and Controls</s1><s2>South Mimms</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>National Institute of Biological Standards and Controls</wicri:noRegion></affiliation></author><author><name sortKey="Matejtschuk, P" sort="Matejtschuk, P" uniqKey="Matejtschuk P" first="P." last="Matejtschuk">P. Matejtschuk</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National Institute of Biological Standards and Controls</s1><s2>South Mimms</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>26 aut.</sZ><sZ>27 aut.</sZ><sZ>28 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>National Institute of Biological Standards and Controls</wicri:noRegion></affiliation></author><author><name sortKey="Gabert, J" sort="Gabert, J" uniqKey="Gabert J" first="J." last="Gabert">J. Gabert</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Biochemistry and Molecular Biology Laboratory ERT MEIDIA, APHM-IFR Jean Roche, Université de la Méditerranée</s1><s2>Marseille</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>29 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université de la Méditerranée</orgName></affiliation></author></analytic><series><title level="j" type="main">Leukemia</title><title level="j" type="abbreviated">Leukemia</title><idno type="ISSN">0887-6924</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Leukemia</title><title level="j" type="abbreviated">Leukemia</title><idno type="ISSN">0887-6924</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Abnormal chromosome C9</term><term>Abnormal chromosome G22</term><term>Amplification</term><term>Bcr-abl protein</term><term>Chromosome translocation</term><term>Freeze</term><term>Gene expression</term><term>Hematology</term><term>Hybrid gene</term><term>Messenger RNA</term><term>Philadelphia chromosome</term><term>Polymerase chain reaction</term><term>Protein kinase</term><term>Real time</term><term>Reference material</term><term>Standardization</term><term>Standards</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Réaction chaîne polymérase</term><term>Matériau référence</term><term>Gène hybride</term><term>Translocation chromosomique</term><term>Chromosome C9 anormal</term><term>Chromosome G22 anormal</term><term>Chromosome Ph1</term><term>Protein kinase</term><term>RNA messager</term><term>Temps réel</term><term>Amplification</term><term>Norme</term><term>Expression génique</term><term>Gel</term><term>Normalisation</term><term>Hématologie</term><term>Gène bcr</term><term>Gène abl</term><term>Protéine bcr-abl</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Norme</term><term>Normalisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Monitoring of BCR-ABL transcripts has become established practice in the management of chronic myeloid leukemia. However, nucleic acid amplification techniques are prone to variations which limit the reliability of real-time quantitative PCR (RQ-PCR) for clinical decision making, highlighting the need for standardization of assays and reporting of minimal residual disease (MRD) data. We evaluated a lyophilized preparation of a leukemic cell line (K562) as a potential quality control reagent. This was found to be relatively stable, yielding comparable respective levels of ABL, GUS and BCR-ABL transcripts as determined by RQ-PCR before and after accelerated degradation experiments as well as following 5 years storage at -20°C. Vials of freeze-dried cells were sent at ambient temperature to 22 laboratories on four continents, with RQ-PCR analyses detecting BCR-ABL transcripts at levels comparable to those observed in primary patient samples. Our results suggest that freeze-dried cells can be used as quality control reagents with a range of analytical instrumentations and could enable the development of urgently needed international standards simulating clinically relevant levels of MRD.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Australie</li><li>Autriche</li><li>Danemark</li><li>Espagne</li><li>Finlande</li><li>France</li><li>Grèce</li><li>Irlande (pays)</li><li>Italie</li><li>Japon</li><li>Pays-Bas</li><li>Royaume-Uni</li><li>Suède</li><li>États-Unis</li></country><region><li>Angleterre</li><li>Aquitaine</li><li>Attique (région)</li><li>Bade-Wurtemberg</li><li>District de Darmstadt</li><li>District de Karlsruhe</li><li>Finlande occidentale</li><li>Grand Londres</li><li>Hesse (Land)</li><li>Hollande-Méridionale</li><li>Nouvelle-Aquitaine</li><li>Piémont</li><li>Provence-Alpes-Côte d'Azur</li><li>Svealand</li><li>Vienne (Autriche)</li><li>Île-de-France</li></region><settlement><li>Athènes</li><li>Bordeaux</li><li>Francfort-sur-le-Main</li><li>Londres</li><li>Mannheim</li><li>Marseille</li><li>Paris</li><li>Rotterdam</li><li>Stockholm</li><li>Turin</li><li>Turku</li><li>Vienne (Autriche)</li></settlement><orgName><li>Université de la Méditerranée</li></orgName></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Saldanhal, J" sort="Saldanhal, J" uniqKey="Saldanhal J" first="J." last="Saldanhal">J. Saldanhal</name></noRegion><name sortKey="Grimwade, D" sort="Grimwade, D" uniqKey="Grimwade D" first="D." last="Grimwade">D. Grimwade</name><name sortKey="Matejtschuk, P" sort="Matejtschuk, P" uniqKey="Matejtschuk P" first="P." last="Matejtschuk">P. Matejtschuk</name><name sortKey="Patel, V" sort="Patel, V" uniqKey="Patel V" first="V." last="Patel">V. Patel</name><name sortKey="Phillips, P" sort="Phillips, P" uniqKey="Phillips P" first="P." last="Phillips">P. Phillips</name></country><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Saldanhal, J" sort="Saldanhal, J" uniqKey="Saldanhal J" first="J." last="Saldanhal">J. Saldanhal</name></region><name sortKey="Beaufils, N" sort="Beaufils, N" uniqKey="Beaufils N" first="N." last="Beaufils">N. Beaufils</name><name sortKey="Cayuela, J M" sort="Cayuela, J M" uniqKey="Cayuela J" first="J.-M." last="Cayuela">J.-M. Cayuela</name><name sortKey="Gabert, J" sort="Gabert, J" uniqKey="Gabert J" first="J." last="Gabert">J. Gabert</name><name sortKey="Macintyre, E" sort="Macintyre, E" uniqKey="Macintyre E" first="E." last="Macintyre">E. Macintyre</name><name sortKey="Mahon, F X" sort="Mahon, F X" uniqKey="Mahon F" first="F.-X." last="Mahon">F.-X. Mahon</name><name sortKey="Silvy, M" sort="Silvy, M" uniqKey="Silvy M" first="M." last="Silvy">M. Silvy</name></country><country name="Australie"><noRegion><name sortKey="Saldanhal, J" sort="Saldanhal, J" uniqKey="Saldanhal J" first="J." last="Saldanhal">J. Saldanhal</name></noRegion><name sortKey="Branford, S" sort="Branford, S" uniqKey="Branford S" first="S." last="Branford">S. Branford</name></country><country name="États-Unis"><noRegion><name sortKey="Arlinghaus, R" sort="Arlinghaus, R" uniqKey="Arlinghaus R" first="R." last="Arlinghaus">R. Arlinghaus</name></noRegion><name sortKey="Sawyers, C" sort="Sawyers, C" uniqKey="Sawyers C" first="C." last="Sawyers">C. Sawyers</name><name sortKey="Wang, J Q" sort="Wang, J Q" uniqKey="Wang J" first="J. Q." last="Wang">J. Q. Wang</name></country><country name="Suède"><region name="Svealand"><name sortKey="Barbany, G" sort="Barbany, G" uniqKey="Barbany G" first="G." last="Barbany">G. Barbany</name></region></country><country name="Italie"><noRegion><name sortKey="Cazzaniga, G" sort="Cazzaniga, G" uniqKey="Cazzaniga G" first="G." last="Cazzaniga">G. Cazzaniga</name></noRegion><name sortKey="Saglio, G" sort="Saglio, G" uniqKey="Saglio G" first="G." last="Saglio">G. Saglio</name><name sortKey="Spinelli, O" sort="Spinelli, O" uniqKey="Spinelli O" first="O." last="Spinelli">O. Spinelli</name></country><country name="Espagne"><noRegion><name sortKey="Gonzalez, M" sort="Gonzalez, M" uniqKey="Gonzalez M" first="M." last="Gonzalez">M. Gonzalez</name></noRegion></country><country name="Finlande"><region name="Finlande occidentale"><name sortKey="Kairisto, V" sort="Kairisto, V" uniqKey="Kairisto V" first="V." last="Kairisto">V. Kairisto</name></region></country><country name="Japon"><noRegion><name sortKey="Miyamura, K" sort="Miyamura, K" uniqKey="Miyamura K" first="K." last="Miyamura">K. Miyamura</name></noRegion></country><country name="Irlande (pays)"><noRegion><name sortKey="Lawler, M" sort="Lawler, M" uniqKey="Lawler M" first="M." last="Lawler">M. Lawler</name></noRegion></country><country name="Autriche"><region name="Vienne (Autriche)"><name sortKey="Lion, T" sort="Lion, T" uniqKey="Lion T" first="T." last="Lion">T. Lion</name></region></country><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Muller, M C" sort="Muller, M C" uniqKey="Muller M" first="M. C." last="Muller">M. C. Muller</name></region><name sortKey="Pfeifer, H" sort="Pfeifer, H" uniqKey="Pfeifer H" first="H." last="Pfeifer">H. Pfeifer</name></country><country name="Danemark"><noRegion><name sortKey="Ostergaard, M" sort="Ostergaard, M" uniqKey="Ostergaard M" first="M." last="Ostergaard">M. Ostergaard</name></noRegion></country><country name="Pays-Bas"><region name="Hollande-Méridionale"><name sortKey="Van Der Velden, V H J" sort="Van Der Velden, V H J" uniqKey="Van Der Velden V" first="V. H. J." last="Van Der Velden">V. H. J. Van Der Velden</name></region></country><country name="Grèce"><region name="Attique (région)"><name sortKey="Zoi, K" sort="Zoi, K" uniqKey="Zoi K" first="K." last="Zoi">K. Zoi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= Characterization of a reference material for BCR-ABL (M-BCR) mRNA quantitation by real-time amplification assays : towards new standards for gene expression measurements
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